Phaseoloideside E, a Novel Natural Triterpenoid Saponin Identified From Entada phaseoloides, Induces Apoptosis in Ec-109 Esophageal Cancer Cells Through Reactive Oxygen Species Generation
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- Mo Shasha
- College of Pharmacy, South Central University for Nationalities, China
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- Xiong Hui
- College of Pharmacy, South Central University for Nationalities, China
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- Shu Guangwen
- College of Pharmacy, South Central University for Nationalities, China
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- Yang Xinzhou
- College of Pharmacy, South Central University for Nationalities, China
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- Wang Jianxia
- College of Pharmacy, South Central University for Nationalities, China
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- Zheng Congyi
- State Key Laboratory of Virology, College of Life Sciences, Wuhan University, China
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- Xiong Wei
- State Key Laboratory of Virology, College of Life Sciences, Wuhan University, China
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- Mei Zhinan
- College of Pharmacy, South Central University for Nationalities, China
書誌事項
- タイトル別名
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- Phaseoloideside E, a Novel Natural Triterpenoid Saponin Identified From <i>Entada phaseoloides</i>, Induces Apoptosis in Ec-109 Esophageal Cancer Cells Through Reactive Oxygen Species Generation
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Phaseoloideside E (PE), a new oleanane-type triterpene saponin, was isolated from the seed kernels of Entada phaseoloides (Linn.) Merr. PE had strong cytotoxic activity against an array of malignant cells. Typical morphological and biochemical features of apoptosis were observed in PE-treated Ec-109 cells. PE induced a dose-dependent increase in the sub-G1 fraction of the cell cycle and DNA fragmentation. Decreases in the mitochondrial membrane potential, SOD activity, and GSH content were also observed. Further investigations revealed that PE reduced the ratio of Bcl-2 to Bax and increased the activities of caspase-3 and -9, but this was prevented by Z-VAD-fmk. PE also induced a decrease of the sub-G1 fraction. Furthermore, PE-induced apoptosis was mediated by up-regulating cellular ROS, which was suppressed by cotreating the cells with N-acetylcysteine (NAC). NAC also attenuated the ratio of sub-G1, the generation of DNA fragmentation and the expression of Bcl-2, Bax, caspase-3, and caspase-9. Interestingly, PE did not up-regulate ROS or induce cell death in untransformed cells. These data showed that PE induces cell death through up-regulation of cellular ROS production. Our investigation provides the scientific basis for the traditional application of this herb and suggests the possibility that PE may be used for a treatment of esophageal carcinoma.<br>[Supplementary materials: available only at http://dx.doi.org/10.1254/jphs.12193FP]
収録刊行物
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 122 (3), 163-175, 2013
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390282680157105280
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- NII論文ID
- 10031185410
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- NII書誌ID
- AA11806667
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- COI
- 1:STN:280:DC%2BC3sjjvFWhtQ%3D%3D
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- ISSN
- 13478648
- 13478613
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- NDL書誌ID
- 024705983
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- PubMed
- 23782641
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可