Curcumin Induces Autophagy via Activating the AMPK Signaling Pathway in Lung Adenocarcinoma Cells
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- Xiao Kui
- Department of Respiratory Medicine, The Second Xiangya Hospital, Central South University, China Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, China
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- Jiang Jiehan
- Department of Respiratory Medicine, Changsha Central Hospital, China
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- Guan Chaxiang
- Department of Physiology, Xiangya School of Medicine, Central South University, China
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- Dong Chunling
- Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, China
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- Wang Guifang
- Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, China
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- Bai Li
- Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, China
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- Sun Jiayuan
- Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, China
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- Hu Chengping
- Department of Respiratory Medicine, Xiangya Hospital, Central South University, China
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- Bai Chunxue
- Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, China
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Abstract
Curcumin is a major yellow pigment and active component of turmeric widely used as dietary spice and herbal medicine. This compound has been reported to be a promising antitumor agent, although the underlying molecular mechanisms are not fully understood yet. In this study, we reported that curcumin inhibited growth of lung adenocarcinoma cells, but had no cytotoxic activity to IMR-90 normal lung fibroblast cells. Curcumin induced autophagy in the A549 human lung adenocarcinoma cell line, evidenced by LC3 immunofluorescence analysis and immunoblotting assays on LC3 and SQSTM1. Moreover, the autophagy inhibitor 3-MA partly blocked the inhibitory effect of curcumin on the growth of A549 cells. Curcumin markedly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetylCoA carboxylase in A549 cells. At last, pharmacological blockade of the AMPK signaling pathway by compound C and genetic disruption of the AMPK signaling pathway with siRNA-mediated AMPKα1 knockdown impaired the autophagy-inducing effect of curcumin. Collectively, our data suggests that curcumin induces autophagy via activating the AMPK signaling pathway and the autophagy is important for the inhibiting effect of curcumin in lung adenocarcinoma cells.
Journal
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 123 (2), 102-109, 2013
The Japanese Pharmacological Society
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Details 詳細情報について
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- CRID
- 1390282680157373056
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- NII Article ID
- 130003362740
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- NII Book ID
- AA11806667
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- COI
- 1:CAS:528:DC%2BC3sXhslertrrM
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- ISSN
- 13478648
- 13478613
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- NDL BIB ID
- 024954565
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- PubMed
- 24048094
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed