Screening of PHA-Producing Bacteria Using Biodiesel-Derived Waste Glycerol as a Sole Carbon Source

DOI Web Site 参考文献26件 オープンアクセス
  • TEEKA Jantima
    Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
  • IMAI Tsuyoshi
    Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
  • CHENG Xuehang
    Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
  • REUNGSANG Alissara
    Department of Biotechnology, Faculty of Technology, Khon Kaen University, Thailand Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Thailand
  • HIGUCHI Takaya
    Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
  • YAMAMOTO Koichi
    Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan
  • SEKINE Masahiko
    Division of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Japan

説明

Different sources of wastewater and soil were used to screen for PHA-producing bacteria using biodiesel-derived waste glycerol as a sole carbon source by the Nile red staining method together with polymer determination. Twelve out of twenty-six isolates from biodiesel-contaminated wastewater consortium were screened for their PHA accumulation ability by cultivation in mineral salt medium supplemented with waste glycerol. The AIK7 isolate was chosen as a potential PHA producer. The PHA production on waste glycerol was examined using pure glycerol as a control substrate. The PHA content of AIK7 isolate cultivated in 10 g/L glycerol could reach 35% cell dry weight in 72 hours from waste glycerol and 33% cell dry weight in 120 hours from pure glycerol cultivation. It can be seen that at this content of waste glycerol, AIK7 isolate is effectively capable of biotransforming glycerol into polymer from low-grade glycerol.

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