Development of a Simple System for the Analysis of Water-Containing Biological Samples by TOF-SIMS

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  • Kanenari Keita
    Graduate School of Electric Engineering and Electronics, Kogakuin University
  • Morita Masato
    Collaborative Open Research Center, Kogakuin University
  • Sakamoto Tetsuo
    Graduate School of Electric Engineering and Electronics, Kogakuin University

Description

In recent years, the needs of mass spectrometry for biological samples have rapidly increased. In this study, in order to analyze water-containing biological samples using time-of-ｆｌight secondary ion mass spectrometry (TOF-SIMS), a rapid-cooling method, sectioning system, and a sample introduction method that could avoid frost formation in plant biological samples were developed. It was confirmed that both frost formation and evaporation of volatile compounds were prevented by a rapid-cooling and new introduction method. Essential elements and H2O could be detected by this new rapid-cooling TOF-SIMS methodology. Therefore, SIMS analysis in the natural state became possible. [DOI: 10.1380/ejssnt.2016.131]

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