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Measurement of <I>Amphidinium</I> sp. Cell Concentration by Fluorometric Method

  • Konishi Atsumi
    The University of Tokyo, Graduate School of Agricultural and Life Science
  • Omasa Kenji
    The University of Tokyo, Graduate School of Agricultural and Life Science
  • Hayashi Masao
    The University of Tokyo, Graduate School of Agricultural and Life Science
  • Masuda Atsunori
    Environmental Plant Engineering Dept., Marine Farm, Yanmar Co., Ltd.
  • Ozawa Tomoko
    Environmental Plant Engineering Dept., Marine Farm, Yanmar Co., Ltd.
  • Tsuda Masashi
    Hokkaido University, Graduate School of Pharmaceutical Science

Bibliographic Information

Other Title
  • 蛍光計測を用いた<I>Amphidinium</I> sp.の濃度測定
  • 蛍光計測を用いたAmphidinium sp.の濃度測定--培養時の光質が増殖速度に及ぼす影響
  • ケイコウ ケイソク オ モチイタ Amphidinium sp ノ ノウド ソクテイ バイヨウジ ノ コウシツ ガ ゾウショク ソクド ニ オヨボス エイキョウ
  • —培養時の光質が増殖速度に及ぼす影響—
  • — Effects of Light Quality on Growth Rate —

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Abstract

In this study, a convenient and stable measurement method of cell concentration of Amphidinium sp. growing in culture solution was developed and it was applied to investigation of effects of different light property on growth rates of the algae. As cell clusters of the algae were observed when the concentration was high, the cell concentration measured with a conventional hemocytometry was underestimated. However, high linear relationships were observed between cell concentration and chlorophyll fluorescence intensity excited at 470 nm light and measured at 680 nm in spite of high concentrations. The relationships were represented as the two regression lines. The one belonged to the algae in the white, blue and red-blue light LED conditions and the other belonged to it in the red light LED conditions. The growth rate of the algae cultured under blue light was much larger than that under red light, and that under red-blue light (red:blue = 4:1) was larger than that under blue light. These results imply that blue lights are necessary for the algae to proliferate rapidly, and even a bit of blue light has the effect of propagate the algae, and chlorophyll fluorescence measurement could be applied to estimation of normally-developed algae cell concentration.

Journal

  • Eco-Engineering

    Eco-Engineering 19 (2), 89-94, 2007

    The Society of Eco-Engineering

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