弱光励起クロロフィル蛍光インダクション画像計測による光阻害の検知

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  • Detection of Photoinhibition in Tomato Leaves with Imaging of Chlorophyll Fluorescence Induction Phenomenon Induced by Weak Actinic Light
  • ジャク ヒカリ レイキ クロロフィル ケイコウ インダクション ガゾウ ケイソク ニ ヨル ヒカリ ソガイ ノ ケンチ

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説明

Chlorophyll fluorescence imaging is a useful tool to assess photosynthetic ability of plant leaf without contact. By applying an actinic light of stable intensity to dark adapted leaf, a dynamic change in chlorophyll fluorescence intensity can be induced. This dynamic change in chlorophyll fluorescence intensity is called “Induction phenomenon”. “Induction method” is a representative way to detect photosynthetic dysfunction in plant leaf by analyzing the induction phenomenon. In this study, we investigated whether imaging of chlorophyll fluorescence induction phenomenon induced by weak actinic light at a PPFD of 10 μmol m-2s-1 can detect photoinhibition, which is caused by strong light irradiation treatments at PPFDs of 500, 1000 and 2000 μmol m-2s-1 for 1 hour. As a result, the photoinhibition in individual tomato leaves caused by the strong light irradiation treatments could be detected by measuring chlorophyll fluorescence induction phenomena that were induced by the weak actinic light. Furthermore, continuous measurement of chlorophyll fluorescence induction phenomena during a relaxation of photoinhibition under dark condition for 24 hours proved that P value, i.e. maximum chlorophyll fluorescence intensity in chlorophyll fluorescence induction phenomenon, can be used as an index of photoinhibition.

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