High-rate Fermentation of Acetate to Methane under Saline Condition by Aceticlastic Methanogens Immobilized in Marine Sediment

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  • KITA Akihisa
    Dept. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University CREST, Japan Science and Technology Agency
  • KOBAYASHI Kounosuke
    Dept. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
  • MIURA Toyokazu
    Dept. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University CREST, Japan Science and Technology Agency
  • OKAMURA Yoshiko
    Dept. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University CREST, Japan Science and Technology Agency
  • AKI Tsunehiro
    Dept. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University CREST, Japan Science and Technology Agency
  • MATSUMURA Yukihiko
    Div. of Energy and Environmental Engineering, Hiroshima University CREST, Japan Science and Technology Agency
  • TAJIMA Takahisa
    Dept. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University CREST, Japan Science and Technology Agency
  • NISHIO Naomichi
    Dept. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
  • NAKASHIMADA Yutaka
    Dept. of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University CREST, Japan Science and Technology Agency

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  • 海洋底泥に固定化された酢酸資化メタノジェンを用いた塩環境下での酢酸からの高効率メタン発酵

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Abstract

High-rate production of methane from acetate using a fixed-bed reactor filled with marine sediment containing halotolerant aceticlastic methanogens and 3 % NaCl was investigated. In continuous culture, the methane production rate increased with increasing dilution rate. The maximum methane production rate of 750 mM d−1 was observed at a dilution rate of 16.2 d−1, which is impossible without immobilization of the cells in the sediment. Using scanning electron microscopy, we observed Methanosaeta-like filamentous microorganisms and Methanosarcina-like coccoid microorganisms in granule-like structures. The results demonstrated that marine sediment is not only a promising microbial resource of halophilic aceticlastic methanogens, but also a supporting material to fix aceticlastic methanogens in a fixed-bed reactor.

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