和牛において見いだされた成長ホルモン遺伝子の多型

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タイトル別名
  • Genetic Variants of the Growth Hormone Gene in Japanese Cattle
  • ワギュウ ニ オイテ ミイダサレタ セイチョウ ホルモン イデンシ ノ タケイ

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抄録

We have previously reported that a nucleotide sequence variant of the bovine growth hormone (bGH) gene at amino acid position 127 can be detected by using the Alu I digestion of a PCR product. To determine the nucleotide sequence of the variant (B type), we amplified and then sequenced 652 by fragments of intron 4/exon 5 of the bGH gene from A and B types of Japanese Black, Holstein, Hereford and Aberdeen Angus cattle. Codon at amino acid position 127 was a GTG for the B type instead of a CTG for the A type, and the other sequence of the B type was in agreement with the A type in the four breeds except for the Japanese Black. In some Japanese Black cattle, a substitution was present at amino acid position 172. This variant (C type) differed from the A and B types by having an ATG at position 172 which caused a replacement of Thr by Met in the deduced amino acid sequence. We designed two oligonucleotide probes for the identification of the variants at position 172. The sequences of the probes were probe A (for Thr172), 5'-CTGCATAAGACGGAGACGTAC-3' and probe C(for Met172), 5'-GTACGTCTCCATCTTATGCAG-3'. The genetypes of the four breeds and Japanese Brown cattle were determined using Alu I digestion for position 127 and a dot hybridization for position 172. Three types of bGH were present in Japanese Black and Japanese Brown cattle ; A type (Leu127, Thr172), B type(Val127, Thr172) and C type (Val121, Met172). Frequencies of the variants were 0.500 (A), 0, 144 (B) and 0.356 (C) in 59 Japanese Black cattle. The C type gene of bGH was not present in the other breeds consisting of 19 Holstein, 10 Hereford and 6 Aberdeen Angus. These results suggest that the C type was caused in the B type gene of ancestral Japanese Black and Japanese Brown cattle. Carcass traits were not affected by the genotypes of the bGH gene.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 65 (4), 340-346, 1994

    公益社団法人 日本畜産学会

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