ブタにおける顆粒膜細胞表層物質の減数分裂休止作用

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タイトル別名
  • Meiotic Arresting Action of the Substance Obtained from Cell Surface of Porcine Ovarian Granulosa Cells
  • ブタ ニ オケル カリュウ マク サイボウ ヒョウソウ ブッシツ ノ ゲンスウ

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抄録

Porcine oocytes isolated from ovarian follicles resumed meiosis in vitro. But resumption of meiosis beyond dictyate stage was prevented by adhesion of oocytes to the granulosa cells layer, and was not prevented by follicular fluid collected from medium sized follicles. Meiotic arresting action of the granulosa cell layer emerged only in the form of adhesion to oocytes. This suggested that meiotic arresting factor is localized on the surface of the cells. Granulosa cells isolated from medium-sized (2-5mm) follicles were incubated with 10mM Tris buffer containing 1M urea and 5mM EDTA (pH 8.3) at 37C for 30min. The substance obtained from cell surface was resolved into two peaks on column chromatography of Sephadex G-25 and the meiotic arresting factor was found in the second peak. Resumption of meiosis of cultivated oocytes was inhibited significantly in the media containing 100μg/ml of lyophilized substance of the second peak. The chemical nature of this factor is still obscure.

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