泌乳増進因子の生物化學的研究

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タイトル別名
  • Studies on the Biological Chemistry of Lactation-stimurating Factors
  • ヒツニュウ ゾウシン インシ ノ セイブツ カガクテキ ケンキュウ ダイ8ホウ Lactoprolactin チュウ ノ tyrosihe ノ ビリョウ ソクテイ ニ ツイテ
  • VIII. On the microdetermination of tryptophane in Lactoprolactin
  • 第8報 Lactoprolactin中のtryptophaneの微量測定について

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The importance of the rôle of tryptophane in the estimation of the minimal molecular weight of proteins has made it desirable to have a practical and accurate method for it's determination in proteins. Among numerous methods for the determination based on colorimetric reaction of tryptophane with aldehyde groups, the author has adopted two procedures, namely, one is a procedure based on a modification of STREPKOV-MAVLIANOV'S method, and another is a procedure based on a modification of SHAW-MCFARLANE'S method.<br>1. The pure preparations of Lactoprolactin have tryptophane of 2.56 per cent by glyoxylic acid method. A modification of the mothod of SHAW-MCFARLANE'S method for tryptphane was described.<br>2. The same preparations of Lactoprolaction contained 1.88 per cent tryptophane by vanillin method. A critical study of the method of STREPKOV-MAVLIANOV'S method for determining tryptophane was made as compared with the glyoxylic acid method.<br>3. Egg albumin A and B contained 1.18 and 1.22 per cent tryptophane respectively by the glyoxylic acid method, and the content of cow's milk was found to average 69.2 mg. per 100 cc. by the same method. However, egg albumin A and B assumed to contain 0.87 and 0.94 per cent tryptophane respectively by the vanillin method, and the content of four layers of egg albumen was between 1.42 to 1.50 per cent by the same method.

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