Estimation of Avian Prolactin by Polyacrylamide Disc Electrophoresis

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  • YAMAMOTO Yoshio
    Department of Animal Reproductin, College of Agriculture and Veterinary Medicine, Nihon University
  • HASHIMOTO Hajime
    Department of Animal Reproductin, College of Agriculture and Veterinary Medicine, Nihon University
  • NAKAJO Seiichi
    Department of Animal Reproductin, College of Agriculture and Veterinary Medicine, Nihon University

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Other Title
  • ディスク電気泳動法による鶏プロラクチン活性の測定法
  • ディスク デンキ エイドウホウ ニヨル ニワトリ プロラクチン カッセイ ノ

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The possibility for the estimation of prolactin in the fowl pituitary by a disc-electrophoresis was investigated. Homogenates of anterior pituitaries from broiler chickens were subjected to the disc-electrophoresis using polyacrylamide gel and divided into 5 segments according to their mobilities. For comparison, NIH-prolactin (ovine) was treated in the same way. Eluates of each segment were assayed for prolactin using a local pigeon crop-gland response. 1. The fastest migrating band was found to be the band which includes prolactin. 2. In the ovine prolactin, a close relationship was found between the dose of eluate and the optical density, and a high correlation coefficient was obtained between the optical density and the crop-gland response. 3. Similar results were also obtained in the eluates of the fastest migrating band in the disc-electrophoresis of the chicken pituitary homogenates. The results indicate that the method using the disc-electrophoresis can be used as a sensitive and simplified method for the quantitative analysis of the fowl prolactin.

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