Rearing of Germfree Chicks in a Vinyl Isolator

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  • ISHIBASHI Teru
    Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
  • KAMETAKA Masao
    Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
  • OZAKI Akira
    Laboratory of Animal Physiology, Institute of Physical and Chemical Research
  • YAMAMOTO Testuzo
    Laboratory of Animal Physiology, Institute of Physical and Chemical Research
  • KANEUCHI Choji
    Laboratory of Animal Physiology, Institute of Physical and Chemical Research
  • MITSUOKA Tomotari
    Laboratory of Animal Physiology, Institute of Physical and Chemical Research

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Other Title
  • ビニルアイソレーター中での無菌ヒナの飼育法
  • ビニルアイソレーターナカ デ ノ ムキン ヒナ ノ シイクホウ エイブン

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Description

A method of rearing germfree chicks in a vinyl isolator was developed, Hatchability of eggs was higher than 95% when eggs were sterilized in a 1.5% mercuric chloride solution on the 2nd day before hatching. Sterility was 93% when 5 to 10 chicks were reared in a vinyl isolator for 2 weeks. Depression of body weight gain was observed in chicks fed diet irradiated at higher than 4 Mrad. However, no effects of irradiation on chick growth were observed when fat and the other components were sterilized separately and mixed before feeding. The growth rates of germfree chicks were higher than those of the conventional chicks which were supplied a diluted solution of fresh cecum feces of young hens with a normal intestinal flora pattern at the first feeding.

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