ブタおよびウシ精子の雌生殖器内培養による呼吸能の増大ならびに精子被覆抗原の除去

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タイトル別名
  • Enhanced Respiration and Reduction of Tetracycline Binding of Boar and Bull Spermatozoa following Incubation in the Female Genital Tract
  • ブタ オヨビ ウシ セイシ ノ メス セイショクキナイ バイヨウ ニヨル コキ

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説明

This experiment was performed to examine the effects of the incubation of boar and bull spermatozoa in the genital tract of estrous sows and cows on the oxygen uptake, motility and tetracycline binding of the spermatozoa in relation to their capacitation. Preincubation of boar spermatozoa with oviduct or uterine rinsings from estrous sows significantly increased the subsequent respiration in phosphate buffer as compared with freshly washed spermatozoa. Preincubation of spermatozoa in the oviducts or uteri isolated from estrous sows and cows resulted in a greater stimulation of respiration than in vitro treatment with rinsings of the female genital tract. Removal of bound tetracycline and retention of sperm motility paralleled the increase in sperm respiration; preincubation of boar spermatozoa in utero for 3h completely removed the bound tetracycline and resulted in a 5 fold increase in the subsequent respiration as compared to freshly washed spermatozoa. The data suggest that a physiological change similar to that reported in the rabbit, also occurs in boar and bull spermatozoa when preincubated in the female genital tract for 3h, and the physiological change may correspond with at least partial capacitation of the spermatozoa.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 46 (9), 531-537, 1975

    公益社団法人 日本畜産学会

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