Production of Hetero-Antibody to Isolated Pig Zonae Pellucidae

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  • ブタ透明帯に対する異種抗体の作製
  • ブタ トウメイタイ ニ タイスル イシュ コウタイ ノ サクセイ エイブン

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Abstract

The antiserum against isolated pig zonae pellucidae was produced in a single goat. The zonae pellucidae were obtained from unfertilized follicular eggs by mincing pig ovaries. The antibody titer was determined by zona precipitate, indirect immunofluorescence and labelled zona precipitate. The antibody titer was gradually increased as the time sequence following immunization and the titer of serum after the nineth injection was 26 by zona precipitate and 210 by indirect immunofluorescence and labelled zona precipitate. The antibody titer did not change after absorption with pig liver, kidney, oviduct and uterus extracts, red blood cells and follicular fluid but clearly declined after absorption with pig isolated zonae pellucidae.

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