走査電子顕微鏡による体外受精マウス卵子透明帯への精子侵入過程の観察

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書誌事項

タイトル別名
  • Scanning Electron Microscopic Observations on the Sperm Penetration through the Zona Pellucida of Mouse Oocytes Fertilized in vitro
  • ソウサ デンシ ケンビキョウ ニヨル タイガイ ジュセイ マウス ランシ トウ

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説明

Superovulated mouse oocytes were fertilized in vitro with pre-incubated cauda epididymal spermatozoa, and the process of sperm penetration through the zona pellucida was studied by scanning electron microscopy. By 5min after insemination, the spermatozoa had passed through the cumulus oophorus, reached the surface of the zona pellucida and had begun to penetrate the zona pellucida. Spermatozoa in the act of penetrating the zona pellucida were frequently found in the oocytes fixed 11min after insemination. The sperm penetration through the zona pellucida was completed within 20min in most oocytes leaving 'sperm penetration hole' on the zona pellucida. The sperm penetration holes were not completely repaired by 4hr after insemination.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 51 (8), 595-601, 1980

    公益社団法人 日本畜産学会

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