Effects of the Time and Temperature of Exposure to Dimethyl Sulphoxide Prior to Freezing on Survival of Frozen-Thawed Rat Embryos

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  • 凍結•融解ラット胚の生存性におよぼすジメチルスルフォキサイドの平衡時間と平衡温度の影響
  • トウケツ ユウカイ ラット ハイ ノ セイゾンセイ ニ オヨボス ジメチルスル

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This study was undertaken to examine effects of the time and temperature of exposure to dimethyl sulphoxide (DMSO) prior to freezing on survival of 8-cell rat embryos after freezing and thawing. Embryos were cooled to-79°C at 0.5°C/min and thawed in air at room temperature. Survival of frozen-thawed 8-cell rat embryos was assessed by their ability to develop into blastocysts during culture. Results are as follows. 1. An exposure to 1.5M DMSO at 30°C for 0.5min prior to freezing conferred higher (P<0.05) survival of frozen-thawed embryos than an exposure at 0°C for the same time. 2. About 42% of embryos survived freezing after only a 0.1min exposure to 1.5M DMSO at 0°C prior to freezing, almost the similar percentage that survived after 5-90 min exposures. To the contrary, a 120min exposure to 1.5M DMSO at 0°C prior to freezing decreased the survival rate of frozen-thawed embryos in comparison with those of embryos frozen after 0.1-90min exposures, although differences were not significant. 3. Regardless of the DMSO concentrations(0.8-2.5M), an exposure of 0.1 and 15min to DMSO at 0°C prior to freezing conferred almost the same protection against freezing damage. In conclusion., DMSO permeation enhances survival of frozen-thawed 8-cell rat embryos, but it is not the sole factor that determines their survival.

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