イノブタ卵巣のΔ5-3β-ステロイド水酸基•脱水素酵素活性

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タイトル別名
  • Histochemical Demonstration of Δ5-3β-Hydroxysteroid Dehydrogenase Activity in the Ovary of the Crossbred of Pig×Wild Boar
  • イノブタ ランソウ ノ デルタ5 3 ベータ ステロイド スイサンキ ダツ ス

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The distribution of enzymeΔ5-3β-hydroxysterold dehydrogenase (3β-HSD) was histochemically studied in the ovary of the crossbred of pig×wild boar during the estrous cycle and at puberty. In antral follicles, 3β-HSD activity was present in theca interna but not in granulosa nor in theca externa. Witn the development of antral follicles, theca interna showed an increased activity of the enzyme with pregnenolone as the substrate, reaching the level of moderate in the follicles over 5mm at proestrus and estrus. Theca interna of atretic antral follicles showed a weaker activity of the enzyme compared to that in theca interna of normal follicles. Corpus luteum showed a stronger activity of the enzyme than other ovarian structures did. The enzyme activity with pregnenolone in corpus luteum was moderate at metestrus and diestrus, decreasing thereafter. Interstitial tissue showed a weak activity of the enzyme, though sometimes moderate, with pregnenolone. The distributional pattern of 3β-HSD activity was similar in puberal and mature animals, appearing in the theca interns of antral follicles and the interstitial tissue. The ovaries of the animals were able to utilize a variety of substrates for the demonstration of 3β-HSD. Of five substrates used, epiandrosterone produced the largest amounts of diformazan deposit, and dehydroepiandrosterone gave a stronger activity than pregnenolone did. These results show that the corpus luteum plays an essential role in the progesterone synthesis in the ovaries of the crossbred of pig×wild boar, and that the theca interna of the antral follicles over 5mm also can contribute to progesterone synthesis. The activity of 3β-HSD was not present in preantral follicles nor in surface epithelium, suggesting the unlikeliness of these structures contributing to the progesterone synthesis.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 56 (4), 353-360, 1985

    公益社団法人 日本畜産学会

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