Conservation of fibroin gene promoter function between the domesticated silkworm <i>Bombyx mori</i> and the wild silkmoth <i>Antheraea yamamai</i>

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  • Sezutsu Hideki
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba
  • Uchino Keiro
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba
  • Kobayashi Isao
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba
  • Tatematsu Ken-ichiro
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba
  • Iizuka Tetsuya
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba
  • Yonemura Naoyuki
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba
  • Tamura Toshiki
    Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences, Tsukuba

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  • Conservation of fibroin gene promoter function between the domesticated silkworm Bombyx mori and the wild silkmoth Antheraea yamamai

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Abstract

To analyze the functional conservation between the fibroin H chain gene promoters in insects of the Bombycidae and Saturniidae, we constructed transgenic silkworms expressing yeast GAL4 under the control of the Bombyx mori or Antheraea yamamai fibroin H chain gene promoter (BmFibH-GAL4 and AyFib-GAL4) and examined their EGFP expression patterns after mating with a homozygous UAS-EGFP strain. Those silkworms carrying the BmFibH-GAL4 and UAS-EGFP constructs showed strict tissue- and stage-specific EGFP expression: expression was observed only in the posterior division of the silk gland after the third day of the fifth instar and then gradually increased as the larvae developed. Similarly, those silkworms carrying AyFib-GAL4 and UAS-EGFP also showed EGFP expression in the posterior division of the silk gland after the third day of the fifth instar; however, some strains expressed EGFP in other tissues as well. Thus, the basic function of the promoter is conserved between the two families, but the regulation of the A. yamamai fibroin gene promoter is seemed to be not as strict as that of the B. mori fibroin H chain promoter when it is used in B. mori.<br>

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