Molecular characterization of aquaporin and aquaglyceroporin in the alimentary canal of Grapholita molesta (the oriental fruit moth): comparison with Bombyx mori aquaporins
-
- Kataoka Naoya
- Laboratory of Insect Physiology, The United Graduate School of Agricultural Sciences, Tottori University
-
- Miyake Seiji
- Laboratory of Insect Physiology, The United Graduate School of Agricultural Sciences, Tottori University
-
- Azuma Masaaki
- Laboratory of Insect Physiology, The United Graduate School of Agricultural Sciences, Tottori University
書誌事項
- タイトル別名
-
- Molecular characterization of aquaporin and aquaglyceroporin in the alimentary canal of <i>Grapholita molesta</i> (the oriental fruit moth) -comparison with <i>Bombyx mori</i> aquaporins
この論文をさがす
抄録
Two different cDNAs similar to Bombyx mori aquaporins (Bommo AQPs) were identified and characterized from the larval alimentary canal of the oriental fruit moth, Grapholita molesta. The first cDNA (AQP-Gra1) encodes a 28 380 Da protein similar to the water-specific AQPs isolated from several liquid-feeding insects such as Haematobia irritans exigua, Aedes aegypti and Cicadella viridis, and from Drosophila melanogaster (DRIP) as well as one of Bommo AQPs (AQP-Bom1). The second cDNA (AQP-Gra2) encodes a 27 929Da protein similar to the other Bommo AQP (AQP-Bom2) and other putative AQPs from D. melanogaster (Aqp17662 and Aqp17664). Functional analysis in Xenopus oocytes microinjected with the capped RNA of G. molesta AQPs revealed that both stimulated osmotic water permeability in a mercury-sensitive manner. The water-specific AQP property in AQP-Gra1 and its higher identity of the deduced amino acid sequence (>70%) with AQP-Bom1 suggests that it is a hindgut-type AQP, likely involved in water-recycling functioning of G. molesta larvae. The AQP-Gra2, most similar (~40% identity) to AQP-Bom2, is considered as a midgut-type and it also increased glycerol and urea permeability. This is the second to demonstrate a member of the aquaglyceroporin family in insects after the first identification of insect aquaglyceroporin from B. mori by our group. The occurrence of two types of AQP, namely aquaporin (water channel) and aquaglyceroporin, appears to be characteristic of fluid-transpoting epithelia in lepidopteran larvae.<br>
収録刊行物
-
- Ｊｏｕｒｎａｌ ｏｆ Ｉｎｓｅｃｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ ａｎｄ Ｓｅｒｉｃｏｌｏｇｙ
-
Ｊｏｕｒｎａｌ ｏｆ Ｉｎｓｅｃｔ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ ａｎｄ Ｓｅｒｉｃｏｌｏｇｙ 78 (2), 2_81-2_90, 2009
社団法人 日本蚕糸学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282680170972928
-
- NII論文ID
- 130004464159
- 10026131796
-
- NII書誌ID
- AA11558849
-
- ISSN
- 18847978
- 13468073
-
- NDL書誌ID
- 10273136
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可