Superoxide Dismutase Activity in the Crude Cell Extract from Streptococcus salivarius subsp. thermophilus 3535

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  • HOSONO Akiyoshi
    Department of Crop and Animal Science, Faculty of Agriculture, Shinshu University
  • SUZUKI Masafumi
    Department of Crop and Animal Science, Faculty of Agriculture, Shinshu University
  • OTANI Hajime
    Department of Crop and Animal Science, Faculty of Agriculture, Shinshu University

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  • Streptococcus sadivarius subsp. thermophilus 3535の菌体抽出液中のスーパーオキシドジスムターゼ活性
  • Streptococcus salivarius subsp.thermoph

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Description

Superoxide dismutase (SOD, EC 1.15.1.1) activity in the crude cell extract prepared from a 24 hour culture of Streptococcus salivarius subsp. thermophilus 3535 was compared with those in the crude cell extracts from six lactic acid bacterial strains for dairy use. It was found that Str. salivarius subsp. thermophilus 3535 was the best producer of SOD among the strains examined. SOD production by this strain rapidly increased in the logarithmic phase of growth, and reached the maximum at 24 hours of incubation. The enzyme had its optimum activity in the neutral pH range from 6.0 to 7.0, and was stable when stood at 60°C for 30 minutes, but remarkably sensitive at 70°C.

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