Screening and Cluster Analysis of Alloantibodies Against Bovine Lymphocyte Antigens (BOLA)

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  • ウシリンパ球抗原(BoLA)に対する同種抗体の検索と特異性の分類
  • ウシ リンパキュウ コウゲン BoLA ニ タイスル ドウシュ コウタイ ノ

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In order to prepare BoLA typing reagents, screening and cluster analysis of alloantibodies against the BoLA were carried out using parous cattle sera and alloimmunized anisera for red cell typing. Sera from 571 parous cows (Holstein : 292, Japanese black: 133, Japanese brown: 72 and Japanese short-horn: 74) were tested by a two-stage microcytotoxicity dye exclusion assay against a panel of 100 lymphocytes (Holstein: 55 and Japanese black: 45). In total, the incidence of cytotoxic antibodies was 67%. The incidence of lymphocytotoxic sera increased gradually with parity (P<0.005). But there were no significant differences between 4 breeds (P>0.50). 153 alloimmunized antisera were tested against 40 lymphocytes. The cytotoxic antibodies were observed in 94% of these sera. Statistical analysis of the reaction patterns utilizing our own computer program revealed 8 clusters of sera which made it possible to detect 8 antigenic specificities. Each cluster consisted of sera from the following breeds; cluster 1 and 4: only Holstein, 7 and 8: mainly from Holstein, 3 and 5: mainly from Japanese black, 6: only Japanese brown, 2: all breeds. There were significant differences in the incidence of 6 antigenic specificities between Holstein and Japanese black. It was suggested that two of the 8 specificities, 7 and 8, showed a linear subgroup relationship. Using these 8 antigens, 55 Holstein were classified into 19 phenotypes, and 45 Japanese black were classified into 14 phenotypes.

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