Effects of medium compositions on Autographa californica nucleopolyhedrovirus replication and cellular gene expression in an Antheraea pernyi cell line

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  • Effects of Medium Compositions on <i>Autographa californica</i> Nucleopolyhedrovirus Replication and Cellular Gene Expression in an <i>Antheraea pernyi</i> Cell Line

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Susceptibility to Autographa californica NPV (AcNPV) of a cell line derived from Antheraea pernyi (AnPe) was drastically improved by changing the culture medium from Sf-900II serum free medium (Sf (−)) to serum-supplemented TC-100 medium (TC (+)). When AnPe cells maintained in Sf (−) and TC (+) were adapted to the mixed medium (Sf (−):TC (+)=1:1) or to serum-supplemented Sf (−), amounts of budded virus and polyhedrin produced in the AcNPV-infected cells as well as, in most cases, expression levels of 5 medium-responsive cellular transcripts, which had been identified by differential display and real-time PCR analyses, resulted in intermediate values between those observed in Sf (−) and TC (+). The results indicated that susceptibility to AcNPV and expression of 5 medium-responsive transcripts in AnPe cells were mainly altered by the balance of components contained in the fetal bovine serum and Sf (−). In addition, expression levels of several medium-responsive transcripts were transiently enhanced after the virus infection, suggesting the possibility that they encode factors promoting and suppressing the AcNPV replication.<br>

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