Effects of Chicken Embryo Extract and Epidermal Growth Factor on Whole-Body Protein Synthesis in Cultured Chicken Embryos

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  • Effects of Chicken Embryo Extract and E

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Abstract

Effects of chicken embryo extract (CEE) and epidermal growth factor (EGF) on whole-body protein synthesis were examined in chicken embryos cultured in vitro. Chicken embryos at 7 days of incubation were transferred into a serum free culture medium containing [3H] phenylalanine with graded levels of either CEE (up to 5%) or EGF(up to 200ng/ml), and cultured in a rotatory whole-embryo culture apparatus for up to 30min. Fractional synthesis rates of whole-body protein were calculated from specific radioactivities of both free and proteinbound phenylalanine in the embryo. The results showed that neither CEE nor EGF significantly increased fractional synthesis rates of whole-body protein in chicken embryos cultured in vitro.

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