生草摂取量指標物質としてのクロロフィルおよびその関連物質の利用上の問題点について

DOI Web Site

書誌事項

タイトル別名
  • Use of Chlorophylls and their Related Substances as Indicators of Pasture Grass Intake
  • セイソウ セッシュリョウ シヒョウ ブッシツ トシテノ クロロフィル オヨビ

この論文をさがす

説明

To determine whether chlorophylls and their related substances were appropriate for indicators of pasture grass intake, amounts of native and converted pheophytins by HCl treatment from chlorophylls (total pheophytins) in feed and feces were measured in two wethers fed fresh oat forage and fresh Italian ryegrass forage. Recoveries of chromogen, which was measured by the absorbance at 410nm and thought to consist of chlorophylls and pheophytins mainly, were 90 and 120% when sheep were fed oat forage and Italian ryegrass forage, respectively. On the other hand, recoveries of total pheophytins were very low. Thin layer chromatographs of intact and acid treated forage showed that absorbances at 410nm were mainly due to chlorophyll a and pheophytin a in both forages and that chlorophyll a and b were perfectly changed by HCl treatment into pheophytin a and b, respectively. There were few chlorophylls in feces. Pheophytins and pigments which did not exist in forages, accounted for about 50% and 35% of the total absorbance of fecal pigments, respectively. The absorption spectra of chlorophyll a and pheophytin a indicated that pheophytin a had 150% higher absorbance than chlorophyll a at 410nm. From these results, it is suggested that chlorophylls and their related substances are changed into absorbable form in the digestive tract and these pigments are not suitable for indicators of pasture grass intake. Chromogen recovery is overestimated by the conversion of chlorophyll a into pheophytin a in the digestive tract and by the existence in fecal pigments which are not found in forages, which suggests that chromogen is not suitable for an indicator of pasture grass intake.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 60 (3), 279-285, 1989

    公益社団法人 日本畜産学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ