Human Serum Albumin Production in Silkworm Bombyx mori Fourth Instar Larvae

DOI Web Site 参考文献21件
  • Kozuma Kazuya
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Kato Masao
    Department of Insect Genetics and Breeding, National Institute of Sericultural and Entomological Science
  • Hamano Kunikatsu
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology

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  • Human Serum Albumin Production in Silkworm <i>Bombyx mori</i> Fourth Instar Larvae

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説明

Recombinant human serum albumin (rHSA) was produced using the recombinant Bombyx mori nucleopolyhedrovirus (rNPV) vector in silkworm larvae. rHSA was purified by ion exchange chromatography, affinity chromatography, and gel filtration chromatography from the whole-body homogenate of infected 4th instar larvae. No impurities were detected in the purified rHSA by reverse-phase chromatography and SDS-polyacrylamide gel electrophoresis. We obtained 3.48 mg of mature rHSA (removed preprosequence) from 24 4th instar larvae on day 4 postinfection (1×102 PFU/larva). These results suggest that efficient rHSA mass production is possible using the rNPV vector and B. mori 4th instar larvae.

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