Identification of an Imidazole Compound-Binding Protein from Diapausing Pharate First Instar Larvae of the Wild Silkmoth <i>Antheraea yamamai</i>

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  • Shimizu Takayuki
    Department of Agro-Bioscience, Faculty of Agriculture, Iwate University
  • Shiotsuki Takahiro
    National Institute of Agrobiological Sciences
  • Seino Atsushi
    National Institute of Agrobiological Sciences
  • An Ying
    Department of Agro-Bioscience, Faculty of Agriculture, Iwate University
  • Kuwano Eiichi
    Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
  • Suzuki Koichi
    Department of Agro-Bioscience, Faculty of Agriculture, Iwate University

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  • Identification of an imidazole compound-binding protein from diapausing pharate first instar larvae of the Wild silkmoth Antheraea yamamai

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A series of 1-substituted and 1, 5-disubstituted imidazoles have been shown to terminate diapause in pharate first instar larvae of the wild silkmoth, Antheraea yamamai, and the gypsy moth, Lymantria dispar. To understand the mode of action for the imidazole compounds, we analyzed imidazole compound (KK-42)-binding proteins by affinity chromatography using a synthetic resin-coupled KK-42 analog. Two binding proteins (KK-42BPs) of 40 and 45 kDa were isolated in soluble fractions prepared from diapausing pharate first instar larvae. The N-terminal amino acid sequence from the first 20 residues was determined for the 45 kDa protein. This 45 kDa protein appeared throughout the periods of pre-diapause and diapause, and it disappeared after the KK-42 application and long period of chilling. It was further demonstrated through specific positive staining with antiserum that the 45 kDa KK-42BP was localized in yolk cells. These results are the first report of a protein associated with artificial hatching in insects.

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