Effects of New Growth Hormone-Releasing Peptide (KP 102) on the Release of Growth Hormone in vitro and in vivo in Cattle

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  • 新しい成長ホルモン放出ペプチド(KP 102)のin vitroおよび in vivoにおけるウシ成長ホルモン放出効果
  • Effects of New Growth Hormone-Releasing

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The effects of KP 102 (also named GHRP-2), a new growth hormone-releasing peptide, on the release of bovine growth hormone (GH) were examined in vitro and in vivo. KP 102 and GH-releasing hormone (GHRH: hGHRH(1-29)-NH2) significantly (P<0.05) stimulated GH release from cultured bovine anterior pituitary cells at doses as low as 10-11M and 10-13M, respectively. When KP102 (10-8M) and GHRH (10-8M or 10-10M) were added together, the additive effects of both peptides on the release of GH were observed (P<0.05). Somatostatin (SS, 10-7M) significantly (P<0.05) blunted GH release induced by KP 102 (10-8M) and GHRH (10-8M). Under the presence of SS, the additive effects of KP 102 and GHRH on the release of GH were also observed (P<0.05). Intravenous injection of 20μg/kg BW KP102 significantly (P<0.05) stimulated GH release in calves. Plasma GH concentrations 10 and 20min after the ivinjection of KP 102 (20μg/kg BW) plus GHRH (0.25μg/kg BW) were significantly (P<0.05) greater than those of either KP 102 alone or GHRH alone. The value of the area under the GH response curve for 60min in GHRH alone and KP 102 plus GHRH was significantly (P<0.05) greater than that of KP 102 alone; however, there were no significant differences between the KP 102 plus GHRH and the GHRH alone groups. These results show that KP 102 stimulates GH release in cattle, and KP 102 interacts with GHRH and SS to release bovine GH.

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