Structural Microheterogeneity of Kefiran from Kefir Grains

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  • MUKAI Takao
    Laboratory of Animal Products Technology, Faculty of Agriculture, Tohoku University
  • TOBA Takahiro
    Laboratory of Animal Products Technology, Faculty of Agriculture, Tohoku University
  • ITOH Takatoshi
    Laboratory of Animal Products Technology, Faculty of Agriculture, Tohoku University
  • ADACHI Susumu
    Laboratory of Animal Products Technology, Faculty of Agriculture, Tohoku University

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  • ケフィール粒由来,ケフィランの構造における微視的不均一性
  • ケフィールリュウ ユライ ケフィラン ノ コウゾウ ニ オケル ビシテキ フキ

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The structure of kefiran from kefir grains was reinvestigated, because 6-O-substituted galactose was detected in methylated derivatives of kefiran, which was not expected to occur from the structure proposed by KOOZMAN3). Hydrolysis of kefiran with Trichoderma reesei cellulase released only glucose and a pentasaccharide. By methylation analysis and 13C-n. m. r. spectroscopy, the pentasaccharide structure was elucidate (1 as β-D-Glc-(1→2)-β-D-Gal-(1→4)-α-D-Gal-(1 →3)-β-D-Gal-(1→4)-D-Glc, and this coincided with the structure proposed by KOOIMAN3). However, methylation analysis of kefiran itself and 13C-n. m. r. spectra suggested the presence of an additional structure including 6-O-substituted galactose. Kefiran is considered to be constructed not only by the structure proposed by KOOIMAN3) but also by another repeating unit including 6-O-substituted galactose.

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