Changes in Intracellular Calcium Concentration and Growth Hormone Release Induced by Nutrients in Primary Cultured Anterior Pituitary Cells of Goats

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  • 培養ヤギ下垂体前葉細胞の細胞内カルシウム•イオン濃度とGH放出に及ぼす栄養素の影響

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Abstract

The effects of glucose, a non-essential amino acid mixture and oleic acid on cytosolic calcium concentration ([Ca2+]i) in individual cells were investigated with a confocal laser scanning microscope in reference to growth hormone (GH) release in caprine anterior pituitary cells cultured for 3d in Dulbecco's modified Eagle's medium. An increase in glucose concentration from 5.5 to 11.0mmol/l in HEPES-buffered saline solution (HBS) caused a slight, but not significant, increase in basal [Ca2+]i and GH release. Administration of amino acids (NEAA; consisting of 7 non-essential amino acids; L-Asp, Gly, L-Ala, L-Ser, L-Pro, L-Asn, and L-Glu; the total concentration was 1.4mmol/l) significantly raised basal [Ca2+]i (86.7% of the total cells) (P<0.05) and GH release (P<0.05). In contrast, oleic acid (10μmol/l) significantly reduced basal [Ca2+]i (83.3% of the total cells)(P<0.05) and GH release (P<0.001). From these findings we conclude that some nutrients such as amino acids and oleic acid have a direct action on caprine anterior pituitary cells to alter the [Ca2+]i level consequently modifying hormone release.

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