Improvement in pHERD vectors to express recombinant proteins tagged with hexahistidine at either the NH<sub>2</sub>- or COOH- terminal in <i>Pseudomonas aeruginosa</i>
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- Iiyama Kazuhiro
- Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture Graduate School, Kyushu University
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- Man Lee Jae
- Laboratory of Silkworm Science, Faculty of Agriculture, Graduate School, Kyushu University
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- Kusakabe Takahiro
- Laboratory of Silkworm Science, Faculty of Agriculture, Graduate School, Kyushu University
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- Yasunaga-Aoki Chisa
- Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture Graduate School, Kyushu University
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- Shimizu Susumu
- Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture Graduate School, Kyushu University
Bibliographic Information
- Other Title
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- Improvement in pHERD vectors to express recombinant proteins tagged with hexahistidine at either the NH₂- or COOH- terminal in Pseudomonas aeruginosa
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Description
We constructed pHERDHis vectors to express polyhistidine-tagged proteins based on the pHERD vectors. The oligonucleotides containing the hexahistidine sequence were designed to be able to introduce the tag at either the NH2- or COOH- terminal. To demonstrate the usefulness of the vectors, a pyocyanin biosynthetic protein, PhzS, was expressed in a pyocyanin-deficient mutant of Pseudomonas aeruginosa. In the mutant strain, both HisPhzS (tag at the NH2- terminal) and PhzSHis (tag at the COOH- terminal) worked as a functional protein and pyocyanin was produced. It was also demonstrated that HisPhzS was purified by standard affinity chromatography.<br>
Journal
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- Journal of Insect Biotechnology and Sericology
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Journal of Insect Biotechnology and Sericology 80 (2), 2_057-2_061, 2011
The Japanese Society of Sericultural Science
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Details 詳細情報について
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- CRID
- 1390282680173002496
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- NII Article ID
- 130004464181
- 10030280362
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- NII Book ID
- AA11558849
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- ISSN
- 18847978
- 13468073
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- NDL BIB ID
- 023417682
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed