Effects of Temperature and Duration of Preservation of Bovine Ovaries in Physiological Saline on The Development of Bovine Embryos Derived from Follicular Oocytes Matured and Fertilized in vitro

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  • ウシ卵巣の生理食塩水中での保存温度および保存時間がウシ体外受精胚発生に及ぼす影響
  • ウシ ランソウ ノ セイリ ショクエンスイチュウ デ ノ ホゾン オンド オヨ

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This study was designed to determine the effects of temperature and duration of preservation of ovaries in physiological saline on the development of bovine embryos derived from follicular oocytes matured and fertilized in vitro. Ovaries obtained in a slaughterhouse were preserved in physiological saline at 38° or 20° for 2h, 4h and 8h. Although it took 2-3h for collection of the ovaries in the slaughterhouse, the end of the collection of ovaries was considered as 0h of preservation. Oocytes surrounded with compact layers of cumulus cells were obtained from follicles of preserved ovaries and cultured for 20-22h. The oocytes were then inseminated with frozen-thawed spermatozoa capacitated with heparin. At 72h after insemination, eggs were evaluated for cleavage and 2- to 16- cell embryos were co-cultured with a cumulus cell monolayer for 3-7 days. The proportions of oocytes that matured to the metaphase II stage of the meiotic division were significantly higher for oocytes from ovaries preserved for 2h (62.8%), 4h (64.2%) and 8h (62.8%) at 20° and those preserved for 2h (67.4%) and 4h (60.7%) at 38°, than those from ovaries preserved for 8h at 38° (33.3%), (P<0.01). The percentages of embryos developed to blastocysts were also significantly higher for embryos derived from ovaries preserved for 2h (13.9%), 4h (13.8%) and 8h (16.6%) at 20° and for 2h (17.5%) and 4h (15.7%) at 38° than those from ovaries preserved for 8h at 38° (1.5%), (P<0.01). It is concluded that ovaries can be preserved in physiological saline for at least 8h at 20° and for 4h at 38° without reducing the fertilizability of oocytes and the capability of embryos to develop to blastocysts.

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