Collagen-Carboxymethyl Dextran Conjugate without Platelet Aggregation

DOI Web Site 被引用文献4件 参考文献42件
  • TOYOKAWA Tetsuya
    Faculty of Agriculture, Tokyo University of Agriculture and Technology Okinawa Industrial Technology Center
  • HATTORI Makoto
    Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • TAKAHASHI Koji
    Faculty of Agriculture, Tokyo University of Agriculture and Technology

この論文をさがす

抄録

Pepsin solubilized collagen-carboxymethyl dextran (PSC-CMD) conjugate was prepared to improve the function of collagen by using cyanogen bromide. The conjugation between PSC and CMD was confirmed by SDS-PAGE, isoelcctric focusing and size-exclusion chromatography. The molar ratio of the conjugate was PSC:CMD=1:2.3. The isoelectric point was shifted from 9.0 to 5.5 by conjugation. Denaturation temperature of the conjugate molecule was 42.2°C, which is 1.9°C higher than that of PSC. The PSC-CMD conjugate maintained self-assembling ability but showed a very slow aggregation as compared with that of PSC. Reconstructed matrix of the conjugate had lower denaturation temperature and random molecular rearrangement structure as compared with PSC matrix. The platelet aggregation inducing ability, analyzed by spectroscopy disappeared.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 70 (4), 219-226, 1999

    公益社団法人 日本畜産学会

被引用文献 (4)*注記

もっと見る

参考文献 (42)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ