Comparative Studies of Lactobacilli and Enterococci Associated with Forage Crops as Silage Inoculants

  • CAI Yimin
    Japan Collection of Microorganisms, The Institute of Physical and Chemical Research
  • KUMAI Sumio
    College of Agriculture, Ehime University
  • ZHANG Janguo
    National Grassland Research Institute
  • BENNO Yoshimi
    Japan Collection of Microorganisms, The Institute of Physical and Chemical Research

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Four selected strains, Lactobacillus casei FG 1, L. plantarum FG 10, Enterococcus faecalis EF 24 and E. faecium EF 62 associated forage crops were characterized, and their effects on silage fermentation was studied. Strains FG 1 and FG 10 were able to grow at lower pH value (3.5) and produce more lactic acid than strains EF 24 and EF 62. Strains FG 1, FG 10, EF 24 and EF 62 were added at a level of 1.0×105 cfu g-1 to alfalfa and Italian. ryegrass silage preparation, and their pH, changes in microbiological composition, organic acid composition, ammonia-nitrogen content and fermentation loss of silage were analyzed after incubation at 25°C for 30 days. During the fermentation process, lactic acid bacteria counts were high in all the additive-treated silages than in the control silages at an early stage of ensiling. Addition of L. casei or L. plantarum effectively inhibited the growth of aerobic bacteria and clostridia more than addition of strains EF 24 or EF 62. The FG 1 and FG 10-treated in each of two silages were well preserved, which had significantly (P<0.05) lower pH values, contents of butyric acid, propionic acid and ammonia-nitrogen, gas-production and dry matter loss, and significantly higher (P<0.05) content of lactic acid than the respective control silage. However, the EF 24 and EF 62-inoculated silages gave similar levels of these components to the control in two kinds of silage. The results confirmed that L. plantarum and L. casei were more effective in producing lactic acid and in improving fermentation quality in the silage environment than E. faecalis and E. faecium.

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  • 日本畜産学会報

    日本畜産学会報 70 (4), 188-194, 1999

    公益社団法人 日本畜産学会

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