書誌事項
- タイトル別名
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- Purification of antioxidant from cherry leaf by high-speed counter-current chromatography and on-line HPLC/DPPH radical scavenging assay
- オンライン HPLC DPPH ラジカル ショウキョ カッセイ シケンホウ オヨビ コウソク コウリュウ クロマトグラフィー ニ ヨル サクラバ チュウ ノ コウサンカ ブッシツ ノ タンリ
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説明
In this study, the identification and purification of antioxidant compound from cherry leaf was proposed by a novel strategy of high-speed countercurrent chromatographic (HSCCC) purification for the efficient and effective discovery of antioxidant from natural product based on on-line HPLC method with radical scavenging assay. To achieve a strategy for HSCCC purification, the antioxidants in cherry leaf extract are identified by on-line HPLC with DPPH radical scavenging assay. Then, the optimal condition of one targeted peak would be investigated for the two-phase solvent system (tert.-butyl methyl ether/acetonitrile/0.1% TFA in water, 2/2/3, V/V/V), and purified by HSCCC. The purification of this antioxidant (3.0mg) form cherry leaf extract (30mg) was performed by HSCCC with optimal two-phase solvent system. Using mass spectrometric and nuclear magnetic resonance analysis, this antioxidant was identified to 3-O-caffeoylquinic acid. Due to the advantages derived from on-line HPLC with DPPH radical scavenging assay and HSCCC technique, a rapid, efficient and effective strategy has been developed for the discovery of antioxidants from natural products.
収録刊行物
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- 日本食品化学学会誌
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日本食品化学学会誌 18 (2), 71-76, 2011
日本食品化学学会
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詳細情報 詳細情報について
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- CRID
- 1390282680173367808
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- NII論文ID
- 110008897458
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- NII書誌ID
- AA11666400
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- ISSN
- 21896445
- 13412094
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- NDL書誌ID
- 11250220
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
