Preparation of Monoclonal Antibody Specific for Incompatible Antibodies Causing Equine Neonatal Isoerythrolysis
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- YOKOHAMA Michinari
- Faculty of Bioindustry, Tokyo University of Agriculture
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- KONDO Tamiaki
- Toa Medical Electronics CO., LTD
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- AKASHIMA Tomohiro
- Faculty of Bioindustry, Tokyo University of Agriculture
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- TERADA Tamotsu
- Hidaka Horse Breeder's Association Shizunai Equine Hospital
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- OHWA Yasuo
- Hidaka Horse Breeder's Association Shizunai Equine Hospital
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- AMANO Takashi
- Faculty of Agriculture, Tokyo University of Agriculture
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Equine neonatal isoerthrolysis (ENI) caused by incompatibilty of blood group alloantigens between a mare and foal has been detected by the indirect antiglobulin test (coombs test) with polyclonal anti-horse γ-globulinan tibody (pAb). However, since this pAb recognizes not only the incompatible antibodies causing ENI (ENI-causing antibody), but other antibodies specific for blood group as specific antigens, the positivity for antibodies in mare serum becomes higher in routine testing.<br>Although newborn foals of positive mares have been prevented from developing serious icterus diseases by withholding the colostrum for a certain period of time, failure of passive antibody transfer (FPT) is a problem. Accordingly, monoclonalanti-horser-globulinantibodies(MonoclonalAbs) with higher specificity for the ENI-causing antibodies were developed by a cell fusion technique.<br>Forty cell lines which produce Monoclonal Abs specific to horse r-globulin were cloned. The Monoclonal Abs from 19 of these cell lines could specifically hemagglutinate the erthrocytes sensitized with the ENI-causing antibodies. The following panel names were given to the 19 Monoclonal Abs : TS-1-TS-5, TS-8-TS-12, A-1-A-3 and G-1-G-6, respectively. The panel of Monoclonal Abs which showed the strongest affinity for the ENI-causing antibodies were TS-4, TS-10, A-1, A-2 and G -1. As a result of clinical testing to prevent ENI in the past three years, the positive rate for mares in the coombs test with Monoclonal Abs (Monoclonal Ab reagents mixed with TS-4 and TS-10 panels) has been significantly decreased in comparison with the results by pAb. These Monoclonal Abs are available for prohylactic for ENI
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 70 (6), 399-407, 1999
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390282680173561472
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- NII論文ID
- 130000742798
- 10020392058
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 4921482
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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