Virulence of phospholipase C mutants of Pseudomonas aeruginosa PAO1 against the silkworm, Bombyx mori
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- Iiyama Kazuhiro
- Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University
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- Chieda Yuuka
- Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University
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- Man Lee Jae
- Laboratory of Silkworm Science, Faculty of Agriculture, Graduate School, Kyushu University
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- Kusakabe Takahiro
- Laboratory of Silkworm Science, Faculty of Agriculture, Graduate School, Kyushu University
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- Yasunaga-Aoki Chisa
- Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University
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- Shimizu Susumu
- Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University
書誌事項
- タイトル別名
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- Virulence of Phospholipase C Mutants of <i>Pseudomonas aeruginosa</i> PAO1 against the Silkworm, <i>Bombyx mori</i>
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To estimate the contribution of phospholipase C (PLC) in the virulence of Pseudomonas aeruginosa against Bombyx mori, PlcB, PlcH, and PlcN mutants were created by the sacB-based counterselection method. Insertion of Ωaac cassette (selection marker, GmR) in plcB, plcH, and plcN was confirmed by Southern hybridization and the mutants were designated as PAO1plcB, PAO1plcH, and PAO1plcN, respectively. Inactivation of PLC genes did not cause growth deficient in vitro. The culture filtrates of PAO1, PAO1plcB, and PAO1plcN had hemolytic activity, but not PAO1plcH. When the PLC mutants were inoculated into the fourth instar of Bombyx mori larvae, all mutants maintained high virulence, indicating that at least inactivation of a single plc gene in P. aeruginosa did not affect the virulence against B. mori. It was different from the results obtained in another Lepidopteran host model, Galleria mellonella.<br>
収録刊行物
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- Journal of Insect Biotechnology and Sericology
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Journal of Insect Biotechnology and Sericology 77 (2), 2_115-2_120, 2008
社団法人 日本蚕糸学会
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詳細情報 詳細情報について
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- CRID
- 1390282680173836544
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- NII論文ID
- 10021221206
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- NII書誌ID
- AA11558849
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- ISSN
- 18847978
- 13468073
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- NDL書誌ID
- 9574043
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可