Purification and properties of double-stranded RNA-degrading nuclease, dsRNase, from the digestive juice of the silkworm, Bombyx mori

DOI Web Site 被引用文献1件 参考文献18件
  • Arimatsu Yuji
    Center for Bioresource Field Science, Faculty of Textile Science, Kyoto Institute of Technology
  • Furuno Tetsuo
    Center for Bioresource Field Science, Faculty of Textile Science, Kyoto Institute of Technology
  • Sugimura Yukio
    Faculty of Textile Science, Kyoto Institute of Technology
  • Togoh Masako
    Center for Bioresource Field Science, Faculty of Textile Science, Kyoto Institute of Technology
  • Ishihara Ryoji
    Center for Bioresource Field Science, Faculty of Textile Science, Kyoto Institute of Technology
  • Tokizane Makoto
    Center for Bioresource Field Science, Faculty of Textile Science, Kyoto Institute of Technology
  • Kotani Eiji
    Faculty of Textile Science, Kyoto Institute of Technology
  • Hayashi Yoshiyuki
    Kinugasa Textile Research Institute, The Kinugasa-kai Foundation
  • Furusawa Toshiharu
    Center for Bioresource Field Science, Faculty of Textile Science, Kyoto Institute of Technology

書誌事項

タイトル別名
  • Purification and Properties of Double-stranded RNA-degrading Nuclease,dsRNase, from the Digestive Juice of the Silkworm, <i>Bombyx mori</i>
公開日
2007
DOI
  • 10.11416/jibs.76.1_57
公開者
一般社団法人 日本蚕糸学会

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説明

A nuclease which degrades double-stranded RNA was purified from the digestive juice of fifth-instar larvae of the silkworm, Bombyx mori, using gel filtration and affinity column chromatography. The enzyme was found to have a molecular weight of 41,000 as estimated by a gel filtration method and detected as a single band with the same molecular weight on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. It degraded double-stranded RNA of cytoplasmic polyhedrosis virus genome, synthetic Poly(I)/Poly(C), Poly(I) and Poly(C). It also digested copolymer Poly(AU), Poly(A) and Poly(U), but showed weak degradation of Poly(A)/Poly(U), Poly(C)/Poly(G), Poly(G) and natural DNA isolated from calf thymus. The pH range wherein the reaction occurred was greater than 7. The purified enzyme did not require Mg2+ to degrade CPV-dsRNA, whereas divalent cations including Mg2+ and Ca2+ were needed to degrade synthetic Poly(I)/Poly(C). The enzyme activity was suppressed by Co2+, Zn2+ and Mn2+.<br>

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