Application of Real Time PCR for the Quantitative Detection of Radiation-induced Genomic DNA Strand Breaks

DOI Web Site 20 References
  • Yamauchi Emiko
    Division of Radiological Protection and Biology, National Institute of Infectious Diseases Department of Biological Production, Tokyo University of Agriculture and Technology
  • Watanabe Ritsuko
    Radiation Effect Analysis Group, Japan Atomic Energy Agency
  • Oikawa Miyoko
    Division of Radiological Protection and Biology, National Institute of Infectious Diseases Division of Science and Technology, Shinshu University
  • Fujimoto Hirofumi
    Division of Radiological Protection and Biology, National Institute of Infectious Diseases
  • Yamada Akinori
    Center of Molecular Biosciences, University of Ryukyus
  • Saito Kimiaki
    Radiation Effect Analysis Group, Japan Atomic Energy Agency
  • Murakami Masahiro
    Radiation Hazard Research Group, National Institute of Radiological Sciences
  • Hashido Kazuo
    Division of Radiological Protection and Biology, National Institute of Infectious Diseases
  • Tsuchida Kozo
    Division of Radiological Protection and Biology, National Institute of Infectious Diseases
  • Takada Naoko
    Division of Radiological Protection and Biology, National Institute of Infectious Diseases
  • Fugo Hajime
    Department of Biological Production, Tokyo University of Agriculture and Technology
  • Tu Zhenli
    College of Animal Sciences, Zhejiang University
  • Maekawa Hideaki
    Division of Radiological Protection and Biology, National Institute of Infectious Diseases

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Abstract

The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with 137Cs γ rays. We verified that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by γ ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.<br>

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