Two Different Constituents of Madder Colors Lack Tumor Promoting or Carcinogenic Potential in a Medium-term Multi-organ Carcinogenesis Bioassay in Rats

DOI Web Site 33 References

Bibliographic Information

Other Title
  • 2種のアカネ色素のラットを用いた中期多臓器発癌性試験での非発癌促進作用ないし非発癌性
  • Two Different Constituents of Madder Co

Search this article

Abstract

The modifying potentials of two madder colors on tumor development were investigated in a medium-term multi-organ carcinogenesis bioassay using male F344 rats. The animals were treated with DEN (100 mg/kg body weight, ip, single injection at the commencement of the study), MNU (20 mg/kg body weight, ip, 4 times during weeks 1 and 2) and DHPN (0.1% in drinking water, during weeks 3 and 4) for multi-organ initiation, and then were given SAN YELLOW AK at dietary levels of 0 (control), 1.5% and 5.0%, SR RED RTM at 2.5% and 5.0%, cochineal (as a negative control) at 5.0%, and S.PB (as a positive control) at 0.05% for 16 weeks. All rats were killed at the end of week 20, and the major organs were carefully examined for preneoplastic and neoplastic lesions. Immunohistochemical demonstration of GST-P positive foci was also employed for quantitative assessment of liver preneoplastic lesion development. No treatment-related changes in clinical signs, body weight, and food consumption were found. Detailed histopathological examination did not demonstrate any enhancement of carcinogenesis with regard to the incidence of preneoplastic and neoplastic lesions in the various target organs/tissues. Quantitative analysis of GST-P positive hepatocytic foci per unit area of liver section did not show any promoting activity on hepatocarcinogenesis. The current results thus revealed that madder colors do not exert tumor promoting or carcinogenic potential in any organ/tissues under the present experimental conditions.

Journal

References(33)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top