ビタミンＡ欠乏系ラットにおけるβ‐カロテン及びカンタキサンチン投与効果の結膜痕跡細胞による研究

廣田 才之, 井上 弘明, 立石 亮, 橋本 秀樹, 富田 純史, 村越 倫明, 二郷 俊朗, 関本 邦敏, 横山 次郎, 舩山 秀樹

doi:10.2740/jisdh.14.194

The reductive metabolic conversion to retinol of beta-carotene and canthaxanthin, in the presence or absence of cholic acid, fed to vitamin A-deficient rats was investigated by the Conjunctival Impression Cytology (CIC) method.<BR>Male Sprague-Dawley rats, 3 weeks old, were raised for 42 days on a Vitamin Adeficient diet prior to the experiment.Beta-carotene, canthaxanthin, in the presence or absence of cholic acid, and retinyl acetate was given for 21 days, and the CIC samples from each group were prepared.<BR>To identify the development and differentiation level of each conjunctival impression cell, the progress of the squamous metaplasia was grading according to six stages, and three ranks; i. e. normal (stage 0, 1), borderline (stage 2, 3), and abnormal (stage 4, 5).<BR>The correlation of the normal CIC population and serum retinol level was also examined. The normal CIC percentage in the beta-carotene group, especially in the presence of the cholic acid group, was higher than that in the control group (2.1 and 2.7 times). The same tendency was observed in the canthaxanthin group, but the normal CIC percentage was lower than the beta-carotene group. The retinyl acetate group also showed higher normal CIC levels compared with the control group (1.9 times). The serum retinol level in each group well reflected each normal CIC percentage.<BR>Together with all of these results, the assessment of the CIC method seems to be useful for identifying the reductive metabolic conversion to retinol by the administration of carotenoids.

ビタミンＡ欠乏系ラットにおけるβ‐カロテン及びカンタキサンチン投与効果の結膜痕跡細胞による研究

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ビタミンＡ欠乏系ラットにおけるβ‐カロテン及びカンタキサンチン投与効果の結膜痕跡細胞による研究

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