Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE×WE) Japanese Quail Embryos
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- Shibuya Kazumoto
- Nippon Institute for Biological Science
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- Mizutani Makoto
- Nippon Institute for Biological Science
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- Sato Kazuo
- Nippon Institute for Biological Science
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- Itabashi Masafumi
- Nippon Institute for Biological Science
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- Nunoya Tetsuo
- Nippon Institute for Biological Science
書誌事項
- タイトル別名
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- Comparative Evaluation of Sex Reversal Effects of Natural and Synthetic Estrogens in Sex Reversal Test Using F1 (AWE*WE) Japanese Quail Embryos
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抄録
Sex reversal effects of 17 beta-estradiol (E2), diethylstilbestrol (DES) and ethynylestradiol (EE2) on male gonads in F1 (AWE×WE) Japanese quail (Corturnix japonica) embryos were comparatively evaluated in a newly developed in vivo screening model called as the sex reversal test. Male and female offspring of F1 (AWE×WE) Japanese quail exhibit exactly wild and albino plumage colors, respectively, ruled by a criss-cross inheritance. The natural and synthetic estrogens were injected into egg white just before the incubation. At 16 days of incubation, embryos were subjected by a complete necropsy and their gonads were grossly observed and examined histopathologically and morphometrically. Grossly, genetic sex confirmed by plumage colors coincided completely with external sex phenotype of the gonads in all embryos of the control group and E2 and DES-treated groups. However, several male embryos with wild plumage in the EE2 2000ng group possessed an ovary-like gonad in the left side and a vestigial right gonad. Histopathologically, E2, DES and EE2 exposures induced a dose-dependent sex reversal effect, i.e. ovotestis development, in the left testis. The left testes showing an ovary-like morphology in the EE2 2000ng group consisted of the most of area replaced with ovarian tissue and the small area of remaining testicular cords. The incidence and morphometric analysis of the ovotestis revealed that the order of potency of sex reversal effect in Japanese quail embryos was EE2>DES>E2. E2, DES and EE2 exposures induced no noticeable changes in the ovaries of any embryos. The present study suggests that the sex reversal test using F1 (AWE×WE) Japanese quail embryo is possible to evaluate feminization effects of endocrine disrupting chemicals with estrogenic activities in avian male embryos.
収録刊行物
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- The Journal of Poultry Science
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The Journal of Poultry Science 42 (2), 119-129, 2005
日本家禽学会
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詳細情報 詳細情報について
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- CRID
- 1390282680182652800
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- NII論文ID
- 10015494685
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- NII書誌ID
- AA11564513
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- ISSN
- 13490486
- 13467395
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- NDL書誌ID
- 7303312
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可