Effect of a Single-Nucleotide Polymorphism in the Cholecystokinin Type A Receptor Gene on Growth Traits in the Hinai-dori Chicken Breed
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- Rikimaru Kazuhiro
- Akita Prefectural Livestock Experiment Station, Japan Graduate School of Agricultural Science, Tohoku University, Japan
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- Takeda Hisato
- National Institute of Livestock and Grassland Science, Japan
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- Uemoto Yoshinobu
- National Livestock Breeding Center, Japan
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- Komatsu Megumi
- Akita Prefectural Livestock Experiment Station, Japan
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- Takahashi Daiki
- Akita Prefectural Livestock Experiment Station, Japan
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- Suzuki Keiichi
- Graduate School of Agricultural Science, Tohoku University, Japan
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- Takahashi Hideaki
- National Institute of Livestock and Grassland Science, Japan
書誌事項
- タイトル別名
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- Effect of a Single-Nucleotide Polymorphism in the <i>Cholecystokinin Type A Receptor</i> Gene on Growth Traits in the Hinai-dori Chicken Breed
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We have previously reported the association between chicken cholecystokinin type A receptor gene (CCKAR) haplotypes and growth traits in an F2 resource population produced by crossing low- and high-growth lines of the Hinai-dori breed. The high-growth line was developed from a low-growth founder from the Preservation Society of the Hinai-dori breed by long-term selection for growth performance at the Akita Prefectural Livestock Experiment Station. In the present study, we determined the effect of a single-nucleotide polymorphism (SNP, AB604331: g.420 C>A) in the 5′-untranslated region of CCKAR on the growth traits of the F2 population. A mismatch amplification mutation polymerase chain reaction assay was developed to distinguish between the 3 genotypes (A/A, A/C, and C/C) in the F2 population, and the effect of the SNP on growth traits was estimated. The data showed that body weight at 10 and 14 weeks of age, and average daily gain between 4 and 10 weeks, 10 and 14 weeks, and 0 and 14 weeks of age in individuals with the A allele was superior to that in those with the C allele. The electrophoretic mobility shift assay was performed to clarify the contribution of the g. 420 C>A SNP in the predicted YY1 binding site. As a result, the YY1 protein showed a stronger binding affinity for g.420 A probe, suggesting the possibility that the SNP affects transcriptional efficiency of the CCKAR. The A allele frequencies in the high- and low-growth lines maintained in fiscal year 2010 were 0.889 and 0.124, respectively. The difference in the allele frequencies of these lines is thought to be caused by long-term selection for growth performance since the probability was too significantly (P<0.01) low to be caused by random genetic drift.
収録刊行物
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- The Journal of Poultry Science
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The Journal of Poultry Science 50 (3), 206-211, 2013
日本家禽学会
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詳細情報 詳細情報について
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- CRID
- 1390282680184045440
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- NII論文ID
- 10031189326
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- NII書誌ID
- AA11564513
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- COI
- 1:CAS:528:DC%2BC3sXhsVCru7nO
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- ISSN
- 13490486
- 13467395
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- NDL書誌ID
- 024706395
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可