制限酵素断片長多型解析とマイクロチップ電気泳動法の抗生物質耐性菌群推定への適用
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- Watanabe Katsuji
- National Agricultural Research Center for Kyushu Okinawa Region
書誌事項
- タイトル別名
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- Application of multiple enzyme restriction fragment length polymorphism analysis and microchip electrophoresis for estimation of antibiotic-tolerant bacterial group
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抄録
A combined method of microchip electrophoresis and multiple enzyme restriction fragment length polymorphism analysis, by which bacterial phylogeny was estimated by comparing the measured multiple enzyme restriction fragment (MERF) to that of the theoretical MERF of various kinds of bacteria, was adapted to monitor an antibiotic-resistant bacterial group in field soils. One hundred and ninety bacteria showing polymyxin B tolerance were newly isolated from experimental field soils where a large amount of liquid livestock feces (600 t/ha/year, 120 t/ha/year) has been applied annually, and neighboring area where feces were not applied (0 t area). In the 0 t area, most bacteria grown on PP agar was Bacillus spp. (80%), which decreased to 21.7% in the 120 t field, and 15.6% in the 600 t field. As the percentages gram negative bacteria, Cytophagales, and Spirochaetales increased to 10.0% in the 120 t field, and 33.3% in the 600 t field, and 7 isolates from the 120 t field and 18 isolates from the 600 t field were ordinary bacteria inhabiting in animals and feces, they originated from livestock. As polymyxin B is bactericidal for gram-negative bacteria, some of the gram-negative bacteria isolated from both the feces-applied fields were supposed to be polymyxin B-resistant bacteria.
収録刊行物
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- 日本農薬学会誌(Journal of Pesticide Science)
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日本農薬学会誌(Journal of Pesticide Science) 33 (3), 249-260, 2008
日本農薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282680185476992
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- NII論文ID
- 110006879103
- 130004445137
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- NII書誌ID
- AA11818622
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- ISSN
- 13490923
- 03851559
- 1348589X
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- NDL書誌ID
- 9625287
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可