Adventitious Shoot Formation, Somatic Embryogenesis and Plantlet Regeneration from In Vitro-Cultured Root Tissue of Japanese Angelica Tree (Aralia elata Seemann)

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  • Furuya Hiroshi
    Hiroshima Prefectural Agriculture Research Center United Graduate School of Agricultural Sciences, Tottori University
  • Hosoki Takashi
    Faculty of Life and Environmental Science, Shimane University United Graduate School of Agricultural Sciences, Tottori University

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Other Title
  • タラノキ（Aralia elata Seemann）培養体由来根組織からの不定芽，不定胚形成および植物体再生
  • 繁殖・育苗 タラノキ(Aralia elata Seemann)培養体由来根組織からの不定芽,不定胚形成および植物体再生
  • ハンショク イクビョウ タラノキ Aralia elata Seemann バイヨウタイ ユライ コン ソシキ カラ ノ フテイ ガ フテイハイケイセイ オヨビ ショクブツタイ サイセイ

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Root segments of plantlets (Aralia elata) grown by in vitro culture, were used as explants and cultured in the dark at 25 °C on MS medium supplemented with growth regulators. About 80-100 % of root segments formed calli on MS medium supplemented with 0.05-4.0 mg/l 2, 4- dichlorophenoxyacetic acid (2, 4-D). After 30 days of culture, calli formed on MS medium containing 0.05 mg/l 2, 4-D, were transferred on hormone-free MS medium and cultured at 25 °C in 16 h light (3,000 lux). Somatic embryogenesis was observed from the calli formed on medium supplemented with more than 0.5 mg/l 2, 4-D. Many somatic embryos and adventitious shoots were induced from the calli and developed into small plantlets by a subculture of 20-30 days interval. After acclimatization on vermiculite, regenerated plantlets were transplanted to a mixture of sandy masa soil/bark compost (3: 1, v/v). Nearly all plants were established and grew in to nursery stocks after 45 days.<br>

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