カロチノイド生合成経路の除草剤標的酵素 Lycopene Cyclase に対する置換アミン類の作用
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- SCHNURR Georg
- Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz
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- BÖGER Peter
- Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz
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- SANDMANN Gerhard
- Biosynthesis Group, Botanical Institute, J. W. Goethe Universität
書誌事項
- タイトル別名
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- Interaction of 2-(4-Methylphenoxy)triethylamine and Related Compounds with Its Herbicide Target in the Carotenoid Biosynthetic Pathway
- Interaction of 2 4 Methylphenoxy trieth
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説明
The carotenogenic enzyme lycopene β-cyclase is found in bacteria, algae and higher plants. The only purified lycopene cyclase to date, originally from the bacterium Erwinia uredovora, was obtained from transformed Escherichia coli and used for cell-free inhibitor studies. Sulfuryl reagents and argininemodifying compounds did not affect enzyme activity. However, 2-(4-methylphenoxy)triethylamine (MPTA), the piperidine carboxylate AMO 1618 (2-isopropyl-4-dimethylamino-5-methylphenyl-1-piperidinecarboxylate methylchloride) and nicotine were effective inhibitors. I50 values for MPTA and nicotine were determined as 12 and 4.8μM, respectively. The type of inhibition of lycopene cyclase by MPTA was non-competitive with respect to the substrate lycopene and also to the cofactor NADH. Except for the sensitivity against sulfuryl reagents, the results on inhibition properties of the E. uredovora lycopene cyclase resemble qualitatively and quantitatively those of the cyanobacterial and higher plant enzyme. Consequently, the expressed and purified lycopene cyclase from this bacterium can be used as a model enzyme to assay potential herbicides which interact with the corresponding plant enzyme.
収録刊行物
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- 日本農薬学会誌(Journal of Pesticide Science)
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日本農薬学会誌(Journal of Pesticide Science) 23 (2), 113-116, 1998
日本農薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282680186491520
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- NII論文ID
- 110001713034
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- NII書誌ID
- AN00196227
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- ISSN
- 03851559
- 13490923
- 1348589X
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- NDL書誌ID
- 4502846
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- データソース種別
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可