Induction and Characterization of Chromosome-doubled Lavandin (Lavandula * intermedia Emeric ex Loisel.) by Colchicine Treatment of Leaf-derived Callus

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  • ラバンジン葉カルスへのコルヒチン処理による倍加個体の作出とその特性
  • Induction and Characterization of Chromosome-doubled Lavandin (Lavandula × intermedia Emeric ex Loisel.) by Colchicine Treatment of Leaf-derived Callus

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Abstract

In order to develop an efficient procedure for chromosome doubling of lavandin, leaf-derived calli were treated with colchicine. Relatively high adventitious shoot formation rates, from 71.8 to 79.3%, were obtained in the medium with 0.05, 0.1 and 0.5% of colchicine, although almost all shoots showed hyperhydricity. However, root formation rates were seriously decreased. Conclusively, 2, 16 and 7 chromosome-doubled plants were obtained from calli treated with 0.05, 0.1 and 0.5% of colchicine, where the regeneration rates were 0.6, 4.3 and 2.0%, respectively. The leaf lengths of chromosome-doubled plants varied widely, and the stomatal size was significantly larger than that of the original. However, both the floral stalks and spikes of chromosome-doubled plants showed dwarfism. Additionally, the essential oil contents in the floral spikes of chromosome-doubled plants significantly decreased. When the crossability is recognized between chromosome-doubled plants and true lavender, these amphidiploids might be interesting materials for improving horticultural traits of lavender.<br>

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