3種の単離植物細胞における脂質, タンパク質および核酸の生合成に対するブタクロルの効果

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  • WANG Yei-Shung
    Department of Agricultural Chemistry, National Taiwan University
  • CHENG Yen-Der
    Department of Agricultural Chemistry, National Taiwan University
  • HSIEH Yar-Ning
    Department of Agricultural Chemistry, National Taiwan University
  • CHEN Yuh-Lin
    Department of Agricultural Chemistry, National Taiwan University

書誌事項

タイトル別名
  • Effect of Butachlor on the Biosynthesis of Lipid, Protein, and Nucleic Acid in Three Isolated Plant Cells
  • 3種の単離植物細胞における脂質,タンパク質および核酸の生合成に対するブタクロルの効果〔英文〕
  • 3シュ ノ タンリ ショクブツ サイボウ ニ オケル シシツ タンパクシツ オ

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抄録

About one half of lipid synthesis was inhibited in Chlorella (46%), tobacco leaf cell (58%), and rice embryo cell (45%) when the isolated cell was incubated at 70μM of Butachlor for 4hr. A thirty percent difference of unincorporated acetate pool size between 70μM of butachlor treated (71%) and untreated (41%) tobacco leaf cells was found in lipid synthesis, but no significant difference was observed in that of Chlorella and rice embryo cells. Within 30min incubation at 70μM of butachlor, protein synthesis was inhibited by 79, 59, and 38% for Chlorella, tobacco leaf cells, and rice embryo cells, respectively, and the inhibition was increased by 92, 95 and 85%, respectively, at 8hr. In contrast to untreated cells, there were 69, 62, and 39% larger pools of absorbed 14C-leucine after treatment of the three cell types with 70μM of butachlor. However, total leucine uptake showed in treated cells was only 52, 37, and 51%, respectively, of that in control. Butachlor inhibited RNA synthesis at 70μM, but at lower concentrations, even stimulation was found.

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