Induction of Polyploids in <i>Vaccinium</i> Using Oryzalin and Colchicine Treatments

DOI Web Site 1 Citations 21 References

Bibliographic Information

Other Title
  • オリザリンおよびコルヒチン処理によるスノキ属植物における倍数体の作出
  • Induction of Polyploids in Vaccinium Using Oryzalin and Colchicine Treatments

Search this article

Description

To establish an efficient polyploid induction method, we carried out in vitro chromosome doubling of multiple shoots in some Vaccinium species. Multiple shoots were aseptically treated with two antimitotic agents, oryzalin and colchicine, at different concentrations and times. After these treatments, the shoots were successively cultured on MW medium containing 5 mg·L−1 zeatin. The ploidy levels of growth shoots were evaluated by both flow cytometry and chromosome counting. The frequency of chromosome-doubled plant production depended on the kinds of antimitotic agent, treatment concentrations and times, and species. Among the treatment conditions tested, oryzalin induced more chromosome doubling than colchicine. Four species of chromosome-doubled plants were obtained with 0.005% 24-hour oryzalin treatment at the following frequencies: V. corymbosum ‘Berkeley’: 23.3%, V. smallii: 5.6%, V. vitis-idaea: 40.0%, V. uliginosum: 57.8%. These results suggest that oryzalin is very effective for polyploid induction of multiple shoots in Vaccinium.<br>

Journal

Citations (1)*help

See more

References(21)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top