Artificial Induction of Tetraploid Plants via Colchicine Treatment and Apical Meristem Culture in <i>Spathiphyllum wallisii</i> Regel ‘Merry’

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  • コルヒチン処理・茎頂培養併用法によるスパティフィラム（<i>Spathiphyllum wallisii</i> Regel‘Merry’）の四倍性個体の誘導
  • Artificial Induction of Tetraploid Plants via Colchicine Treatment and Apical Meristem Culture in Spathiphyllum wallisii Regel ^|^lsquo;Merry^|^rsquo;

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Abstract

The induction of tetraploidy in Spathiphyllum wallisii Regel ‘Merry’ was successfully achieved using whole tuber colchicine treatment along with apical meristem culture to produce new cultivars or polyploidy breeding materials. The whole tubers, from which expanded and unexpanded leaves were removed, were treated with 0, 0.1, 1.0 and 10.0 mM colchicine in 1% (v/v) DMSO solution for 12, 24 or 48 h, respectively. Three chimeric plantlets, that were both diploid and tetraploid, checked with a flow cytometric procedure, were obtained with the 10.0 mM colchicine treatment. To obtain a complete tetraploid plantlet, shoot apical meristems of three chimeras were cut and cultured in vitro. The regenerated plantlets obtained through multiple buds were successfully acclimatized. Confirmation of the ploidy level in the developed plantlets by flow cytometry indicated that a complete tetraploid plantlet was obtained. In comparison with diploid plantlets, the tetraploid plantlet showed some morphological differences: enlargement of guard cells, thickening of floral stem, spadix and spathe, ovateness in spathe and leaf and increment of leaf width/leaf length ratio.<br>

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