Analysis of the Cellular Stress Response in MCF10A Cells Exposed to Combined Radio Frequency Radiation

DOI PDF Web Site PubMed 参考文献28件
  • KIM Han-Na
    Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences
  • HAN Na-Kyung
    Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences School of Life Sciences and Biotechnology, Korea University
  • HONG Mi-Na
    Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences
  • CHI Sung-Gil
    School of Life Sciences and Biotechnology, Korea University
  • LEE Yun-Sil
    College of Pharmacy & Division of Life Science and Pharmaceuticals, Ewha Womans University
  • KIM Taehong
    Electromagnetic Research Center, Chungnam National University
  • PACK Jeong-Ki
    Electromagnetic Research Center, Chungnam National University
  • CHOI Hyung-Do
    EM Environment Research Team, Electronics and Telecommunications Research Institute
  • KIM Nam
    School of Electrical and Computer Engineering, Chungbuk National University
  • LEE Jae-Seon
    Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences

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Exposure to environmental stressors can be measured by monitoring the cellular stress response in target cells. Here, we used the cellular stress response to investigate whether single or combined radio frequency (RF) radiation could induce stress response in human cells. Cellular stress responses in MCF10A human breast epithelial cells were characterized after exposure to 4 h of RF radiation [code division multiple access (CDMA) or CDMA plus wideband CDMA (WCDMA)] or 2 h RF radiation on 3 consecutive days. Specific absorption rate (SAR) was 4.0 W/kg for CDMA signal alone exposure and 2.0 W/kg each, 4.0 W/kg in total for combined CDMA plus WCDMA signals. Expression levels and phosphorylation states of specific heat shock proteins (HSPs) and mitogen-activated protein kinases (MAPKs) were analyzed by Western blot. It was found that HSP27 and ERK1/2 phosphorylations are the most sensitive markers of the stress response in MCF10A cells exposed to heat shock or ionizing radiation. Using these markers, we demonstrated that neither one-time nor repeated single (CDMA alone) or combined (CDMA plus WCDMA) RF radiation exposure significantly altered HSP27 and ERK1/2 phosphorylations in MCF10A cells (p > 0.05). The lack of a statistically significant alteration in HSP27 and ERK1/2 phosphorylations suggests that single or combined RF radiation exposure did not elicit activation of HSP27 and ERK1/2 in MCF10A cells.

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